(A, B) RNA in situ hybridization of stage 14 (A) and stage 16 (B) embryos with the ouib antisense RNA probe. Dorsal views are shown. ouib signal was detected in the primordia of PG cells (arrows). An image with sense RNA probe is shown in S1 Fig (C, D) in situ hybridization of third instar larval brain-ring gland complexes with the ouib antisense (C) and sense (D) RNA probes. ouib signal is detected in the ring gland including the PG cells (arrow). (E) The expression levels of ouib in several tissues quantified by qRT-PCR (N = 3). Total RNA was prepared from wandering third instar larvae. BR, brain; RG, ring gland; ID, imaginal disc; IN, intestine; FB, fat body; SG, salivary gland. Error bars indicate the .